Custom Antibody Purification and Labeling Services

Inquiries to:

Kristin Byrne
508-435-2080 x3322
800-435-2080 x3322
kristinb@qcb.com
Fax: 508-435-8508

Blood serum is often monitored for biochemical markers of disease and specific antibody production. The high concentration of albumin (50-70% of total serum proteins) and IgG (10-25% of total serum proteins) can interfere with the resolution and sensitivity of low concentration proteins. Protein A, Protein G and Peptide Specific Anti-antibody Affinity Columns are three common methods for antibody purification and/or IgG removal from serum or ascites fluid.

Protein A and Protein G have an affinity for the Fc portion of the IgG molecule. This interaction separates IgG from other immunoglobulins, contaminants and serum proteins. Peptide Specific Anti-antibody Columns bind the F(ab)2 portion of IgG and purify antibodies specific to the antigen. These columns can also be designed to remove antibodies to histidine tagged portions of recombinant proteins, to remove contaminating or cross-reacting antibodies or to purify and separate multiple phospho-specific sites on a protein.

QCB utilizes all three purification techniques in IgG purification or depletion from serum and ascites fluid. We customize purification methods to meet specific product requirements and research budgets. Please call our technical immunology group to discuss your research needs.

Affinity purification column synthesis

Customer supplied peptide or protein is conjugated to column resin. Unbound sites on the resin are blocked and column is equilibrated for optimal binding.

Affinity purification of antibody

Antisera is purified over a custom affinity purification column. Specific antibodies bind to the column and are eluted resulting in approximately 4-6mg of highly specific, purified antibody per 50ml of purified serum.

Single-step phospho-specific antibody purification

QCB has developed a fast, single-step Phospho-antibody Affinity Purification Column using proprietary technology. A customized phospho-peptide is conjugated to a thiol-coupling resin and supplied in a ready-to-use 10mm column. Our purification steps are simple, fast and reduce antibody loss due to over processing.

1. Equilibrate column with binding buffer
2. Incubate serum with column
3. Wash column to remove non-specific proteins
4. Elute phospho specific antibody
5. Re-equilibrate column for future use

Protein A and G purification

Protein A and Protein G affinity columns are customized to meet your product requirements. Columns can be used to purify or deplete IgG from serum or ascites fluid. Prices are custom and relate to the amount of IgG requested or the volume of serum or ascites fluid processed.

PSSA or CSSA purification

Two columns are prepared using the phospho and non-phospho peptides (or, the cleavage-site and spanning peptides). Antisera is processed over the phospho column and bound material is eluted. The eluted material is processed over the non-phospho column to remove any pan or non-phospho antibodies.

Multiple antibody affinity purifications

One column is made with each of the immunized peptides in order to purify multiple specific antibodies from one pool of serum. One pool may be processed over individual peptide columns several times depending upon initial antibody concentration.

Fragmentation, Biotinylation and other labels are available upon request.