Phospho Site Specific
Antibody (PSSA) Package
Project Services
- Antigen design
- Technical support throughout your project
- Synthesis of phospho (P) peptide up to 18 amino acids
- Synthesis of non-phospho (NP) pep up to 18 amino acids
- Purification of the P peptide to ~90%
- Purification of the NP peptide to ~80%
- Mass Spec & HPLC verification of peptide sequences
- 4-6 mg each of phospho & non phospho peptide shipped to customer
- Conjugation of the peptide for immunization
- Polyclonal antibody production in two rabbits
- Prebleeds, 5 immunizations and 4 production bleeds
(yields 180-200 ml serum)
- ELISA testing on bleeds 2 & 3
Serum tested over both P and NP peptides
- Characterization and evaluation Period (30 days)
- Affinity purification (yield: ~2-4 mg purified antibody)
Preparation of both P and NP columns
Negative preabsorbtion over the NP column
Purification of NP depleted serum over the P column
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Cleavage Site Specific
Antibody (CSSA) Package
Project Services
- Antigen design
- Technical Support throughout your project
- Synthesis of cleavage site peptide, up to 18 amino acids
- Synthesis of spanning peptide (SP), up to 18 amino acids
- Purification of the cleavage site peptide to ~90%
- Purification of the spanning peptide to >80%
- Mass Spec & HPLC verification of peptide sequences
- 4-6 mg of cleavage site peptide shipped to customer
- Conjugation of the cleavage peptide for immunization
- Polyclonal antibody production in two rabbits
- Prebleeds, 5 immunizations and 4 production bleeds
(yields 180-200 ml serum)
- ELISA testing on bleeds 2 & 3
Serum tested over both cleavage site and SP peptides
- Characterization and Evaluation Period (30 days)
- Affinity purification (yield: ~3-6 mg purified antibody)
Preparation of both cleavage and SP columns
Negative preabsorbtion over the SP column
Purification of spanning peptide depleted serum over
cleavage product column
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